The enzyme telomerase provides a mechanism that ensures chromosome stability in both normal and neoplastic cells. This reference covers methods of dentral importance in the analysis of telomerase function and activity for all cell and molecular biologists. These range from the standard telomerase activity assay through to many more novel approaches such as the use of real-time PCR, in-situ PCR and non-PCR based assays of telomerase activity. Also included are protocols for the study of telomeres themselves and methods for the identification of regulators of telomerase function.
The enzyme telomerase provides a mechanism that ensures chromosome stability in both normal and neoplastic cells. This reference covers methods of dentral importance in the analysis of telomerase function and activity for all cell and molecular biologists. These range from the standard telomerase activity assay through to many more novel approaches such as the use of real-time PCR, in-situ PCR and non-PCR based assays of telomerase activity. Also included are protocols for the study of telomeres themselves and methods for the identification of regulators of telomerase function.